By Mr Ralf Kohler

Journal articles

Kroh S; Pascual-Reguant A; Manukyan A; Uecker R; Günther R; Philipsen L; Koehler R; Mex P; Landthaler M; Niesner RA; Hauser AE, 2026, 'Spatial and Phenotypic Heterogeneity of ILC Subsets in Mouse Lung Under Type 2 Inflammatory Conditions', European Journal of Immunology, 56, http://dx.doi.org/10.1002/eji.70223

Shakespeare T; Seehra RS; Flores Rodriguez N; Atuanya N; Sheard TMD; Köhler R; Bose D; Wunderley L; Woodman P; Ciani B; Jayasinghe I, 2026, 'Mapping Epidermal Growth Factor Receptor-1 Sorting Domains in Endosomes with a Calibrated Three-Dimensional Expansion Microscopy Toolkit', ACS Nano, 20, pp. 10074 - 10089, http://dx.doi.org/10.1021/acsnano.6c00277

Mertens TF; Liebheit AT; Ehl J; Köhler R; Rakhymzhan A; Woehler A; Katthän L; Ebel G; Liublin W; Kasapi A; Triantafyllopoulou A; Schulz TJ; Niesner RA; Hauser AE, 2024, 'MarShie: a clearing protocol for 3D analysis of single cells throughout the bone marrow at subcellular resolution', Nature Communications, 15, pp. 1764, http://dx.doi.org/10.1038/s41467-024-45827-6

Rakhymzhan A; Fiedler AF; Günther R; Domingue SR; Wooldridge L; Leben R; Cao Y; Bias A; Roodselaar J; Köhler R; Ulbricht C; Heidelin J; Andresen V; Beckers I; Haibel A; Duda G; Hauser AE; Niesner RA, 2024, 'Optimized intravital three-photon imaging of intact mouse tibia links plasma cell motility to functional states', iScience, 27, pp. 110985, http://dx.doi.org/10.1016/j.isci.2024.110985

Mothes R; Pascual-Reguant A; Koehler R; Liebeskind J; Liebheit A; Bauherr S; Philipsen L; Dittmayer C; Laue M; von Manitius R; Elezkurtaj S; Durek P; Heinrich F; Heinz GA; Guerra GM; Obermayer B; Meinhardt J; Ihlow J; Radke J; Heppner FL; Enghard P; Stockmann H; Aschman T; Schneider J; Corman VM; Sander LE; Mashreghi MF; Conrad T; Hocke AC; Niesner RA; Radbruch H; Hauser AE, 2023, 'Distinct tissue niches direct lung immunopathology via CCL18 and CCL21 in severe COVID-19', Nature Communications, 14, http://dx.doi.org/10.1038/s41467-023-36333-2

Treimer W; Köhler R, 2021, 'Determination of the Spatial Resolution in the Case of Imaging Magnetic Fields by Polarized Neutrons', Applied Sciences, 11, pp. 6973, http://dx.doi.org/10.3390/app11156973

Du W; Lenz D; Köhler R; Zhang E; Cendon C; Li J; Massoud M; Wachtlin J; Bodo J; Hauser AE; Radbruch A; Dong J, 2021, 'Rapid Isolation of Functional ex vivo Human Skin Tissue-Resident Memory T Lymphocytes', Frontiers in Immunology, 12, pp. 624013, http://dx.doi.org/10.3389/fimmu.2021.624013

Pascual-Reguant A; Köhler R; Mothes R; Bauherr S; Hernández DC; Uecker R; Holzwarth K; Kotsch K; Seidl M; Philipsen L; Müller W; Romagnani C; Niesner R; Hauser AE, 2021, 'Multiplexed histology analyses for the phenotypic and spatial characterization of human innate lymphoid cells', Nature Communications, 12, pp. 1737, http://dx.doi.org/10.1038/s41467-021-21994-8

Dornieden T; Sattler A; Pascual-Reguant A; Ruhm AH; Thiel LG; Bergmann YS; Thole LML; Köhler R; Kühl AA; Hauser AE; Boral S; Friedersdorff F; Kotsch K, 2021, 'Signatures and Specificity of Tissue-Resident Lymphocytes Identified in Human Renal Peritumor and Tumor Tissue', Journal of the American Society of Nephrology, 32, pp. 2223 - 2241, http://dx.doi.org/10.1681/asn.2020101528

Riedel R; Addo R; Ferreira-Gomes M; Heinz GA; Heinrich F; Kummer J; Greiff V; Schulz D; Klaeden C; Cornelis R; Menzel U; Kröger S; Stervbo U; Köhler R; Haftmann C; Kühnel S; Lehmann K; Maschmeyer P; McGrath M; Naundorf S; Hahne S; Sercan-Alp Ö; Siracusa F; Stefanowski J; Weber M; Westendorf K; Zimmermann J; Hauser AE; Reddy ST; Durek P; Chang H-D; Mashreghi M-F; Radbruch A, 2020, 'Discrete populations of isotype-switched memory B lymphocytes are maintained in murine spleen and bone marrow', Nature Communications, 11, pp. 2570, http://dx.doi.org/10.1038/s41467-020-16464-6

Kriegel FL; Köhler R; Bayat-Sarmadi J; Bayerl S; Hauser AE; Niesner R; Luch A; Cseresnyes Z, 2018, 'Morphology-Based Distinction Between Healthy and Pathological Cells Utilizing Fourier Transforms and Self-Organizing Maps', Journal of Visualized Experiments, http://dx.doi.org/10.3791/58543-v

Kriegel FL; Köhler R; Bayat-Sarmadi J; Bayerl S; Hauser AE; Niesner R; Luch A; Cseresnyes Z, 2018, 'Morphology-Based Distinction Between Healthy and Pathological Cells Utilizing Fourier Transforms and Self-Organizing Maps', Journal of Visualized Experiments, pp. 58543, http://dx.doi.org/10.3791/58543

Kriegel FL; Köhler R; Bayat-Sarmadi J; Bayerl S; Hauser AE; Niesner R; Luch A; Cseresnyes Z, 2018, 'Cell shape characterization and classification with discrete Fourier transforms and self-organizing maps.', Cytometry A, 93, pp. 323 - 333, http://dx.doi.org/10.1002/cyto.a.23279

Holzwarth K; Köhler R; Philipsen L; Tokoyoda K; Ladyhina V; Wählby C; Niesner RA; Hauser AE, 2018, 'Multiplexed fluorescence microscopy reveals heterogeneity among stromal cells in mouse bone marrow sections', Cytometry Part A, 93, pp. 876 - 888, http://dx.doi.org/10.1002/cyto.a.23526

Radbruch H; Mothes R; Bremer D; Seifert S; Köhler R; Pohlan J; Ostendorf L; Günther R; Leben R; Stenzel W; Niesner RA; Hauser AE, 2017, 'Analyzing Nicotinamide Adenine Dinucleotide Phosphate Oxidase Activation in Aging and Vascular Amyloid Pathology', Frontiers in Immunology, 8, pp. 844, http://dx.doi.org/10.3389/fimmu.2017.00844

Preprints

Riedel R; Addo R; Ferreira-Gomes M; Heinz GA; Heinrich F; Kummer J; Greiff V; Schulz D; Klaeden C; Cornelis R; Menzel U; Kröger S; Stervbo U; Köhler R; Haftmann C; Kühnel S; Lehmann K; Maschmeyer P; McGrath M; Naundorf S; Hahne S; Sercan-Alp Ö; Siracusa F; Stefanowski J; Weber M; Westendorf K; Zimmermann J; Hauser AE; Reddy ST; Durek P; Chang H-D; Mashreghi M-F; Radbruch A, Discrete populations of isotype-switched memory B lymphocytes are maintained in murine spleen and bone marrow, http://dx.doi.org/10.1101/825224

Rakhymzhan A; Fiedler AF; Günther R; Domingue S; Wooldridge L; Leben R; Cao Y; Bias A; Roodselaar J; Köhler R; Ulbricht C; Heidelin J; Andresen V; Beckers I; Haibel A; Duda G; Hauser AE; Niesner RA, In vivo optimized three-photon imaging of intact mouse tibia links plasma cell motility to functional states in the bone marrow, http://dx.doi.org/10.1101/2023.12.16.571998

Mothes R; Pascual-Reguant A; Koehler R; Liebeskind J; Liebheit A; Bauherr S; Dittmayer C; Laue M; von Manitius R; Elezkurtaj S; Durek P; Heinrich F; Heinz GA; Guerra GM; Obermayer B; Meinhardt J; Ihlow J; Radke J; Heppner FL; Enghard P; Stockmann H; Aschman T; Schneider J; Corman V; Sander LE; Mashreghi M-F; Conrad T; Hocke A; Niesner RA; Radbruch H; Hauser AE, Local CCL18 and CCL21 expand lung fibrovascular niches and recruit lymphocytes, leading to tertiary lymphoid structure formation in prolonged COVID-19, http://dx.doi.org/10.1101/2022.03.24.22272768

Shakespeare T; Seehra RS; Rodriguez NF; Atuanya N; Sheard TMD; Köhler R; Bose D; Woodman P; Ciani B; Jayasinghe I, Mapping EGFR1 sorting domains in endosomes with a calibrated 3D expansion microscopy toolkit, http://dx.doi.org/10.1101/2025.10.01.678490

Köhler R; Hurley ME; White E; Jayasinghe I, Structural and functional heterogeneity in cardiac RyR signalling nanodomains revealed with quantitative single molecule mapping toolkit, http://dx.doi.org/10.64898/2026.07.22.740006

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