By Dr Chai Ann Ng

Book Chapters

Vandenberg JI; Ng CA; Mann SA; Perry MD, 2014, 'Voltage-Gated Potassium Channels (Kv10-Kv12)', in Reference Module in Biomedical Research, http://dx.doi.org/10.1016/B978-0-12-801238-3.04809-1

Journal articles

Thomson KL; Jiang C; Richardson E; Westphal DS; Burkard T; Wolf CM; Vatta M; Harrison SM; Ingles J; Bezzina CR; Kroncke BM; Vandenberg JI; Ng CA, 2024, 'Clinical interpretation of KCNH2 variants using a robust PS3/BS3 functional patch-clamp assay', Human Genetics and Genomics Advances, 5, pp. 100270, http://dx.doi.org/10.1016/j.xhgg.2024.100270

Austin R; Brown JS; Casauria S; Madelli EO; Mattiske T; Boughtwood T; Metke A; Davis A; Horton AE; Winlaw D; Das D; Soka M; Giannoulatou E; Rath EM; Haan E; Blue GM; Vohra J; Atherton JJ; van Spaendonck-Zwarts K; Cox K; Burnett L; Wallis M; Haas M; Quinn MCJ; Pachter N; Poplawski NK; Stark Z; Bagnall RD; Weintraub RG; Pantaleo S-J; Lunke S; De Fazio P; Thompson T; James P; Chang Y; Fatkin D; Macciocca I; Ingles J; Dunwoodie SL; Semsarian C; McGaughran J; Ades L; Enriquez A; McLean A; Smyth R; Alankarage D; Fatkin D; McNamara J; Soka M; Morgan almog ; Fear V; Medi C; Stark Z; Al-Shinnag M; Fine M; Metke A; Sy R; Atherton JJ; Finlay K; Milnes D; Tang D; Austin R; Garza D; Milward M; Taylor J; Bagnall RD; Giannoulatou E; Morrish A; Taylor S; Barnett C; Gongolidis L; Morwood J; Tchan M; Blue GM; Gray B; Mountain H; Thompson T; Bodek S; Greer C; Mowat D; Thorpe J; Boggs K; Haan E; Ng C-A; Trainer A; Bogwitz M; Haas M; Nowak N; Trivedi G; Boughtwood T; Hanna B; Martinez NN; Valente G; Bray A; Harvey R; Ohanian M; van Spaendonck-Zwarts K; Brion M-J; Hayward J; O’Sullivan S; Vandenberg J; Brown J; Herrera C; Overkov A; Verma K; Richardson RB; Hill A; Pachter N; Vidgen M; Burnett L; Hollingsworth G; Patel C; Vohra J; Burns C; Hollway G; Perrin M; Waddel-Smith K; Cao M; Horton AE; Perry M; Wallis M; Carr W; Howting D; Pflaumer A; Weintraub RG; Casauria S; Ingles J; Phillips P; Wilson M; Chalinor H; Isbister J; Phuong T; Winlaw D; Chang Y; Jackson M; Pope-Couston R; Worgan L; Chapman G; James P; Poplawski NK; Wornham L; Charitou T; Jane-Pantaleo S; Punni P; Wu K; Chong B; Johnson R; Quinn MCJ; Yeates L; Collins F; Kelly A; Quinn M; Zentner D; Correnti G; King-Smith S; Rajagopalan S; Cox K; Kirk E; Raju H; Cunningham F; Kummerfeld S; Rath EM; Das D; Lassman T; Regan M; Davis J; Lipton J; Rogers J; Davis A; Lunke S; Ryan M; De Fazio P; Macciocca I; Sandaradura S; de Silva M; MacIntyre P; Schonrock N; Den Elzen N; Madelli EO; Scuffham P; Devery S; Mallawaarachchi A; Semsarian C; Dobbins J; Mansour J; Sherburn I; Dunwoodie SL; Martin E; Sherlock M-C; Dwyer N; Mathew J; Singer E; Elbracht-Leong S; Mattiske T; Smerdon C; Elliott D; McGaughran J; Smith J, 2024, 'A multitiered analysis platform for genome sequencing: Design and initial findings of the Australian Genomics Cardiovascular Disorders Flagship', Genetics in Medicine Open, 2, pp. 101842 - 101842, http://dx.doi.org/10.1016/j.gimo.2024.101842

Kaizer AM; Winbo A; Clur SAB; Etheridge SP; Ackerman MJ; Horigome H; Herberg U; Dagradi F; Spazzolini C; Killen SAS; Wacker-Gussmann A; Wilde AAM; Sinkovskaya E; Abuhamad A; Torchio M; Ng CA; Rydberg A; Schwartz PJ; Cuneo BF, 2023, 'Effects of cohort, genotype, variant, and maternal β-blocker treatment on foetal heart rate predictors of inherited long QT syndrome', Europace, 25, http://dx.doi.org/10.1093/europace/euad319

Copier JS; Bootsma M; Ng CA; Wilde AAM; Bertels RA; Bikker H; Christiaans I; van der Crabben SN; Hol JA; Koopmann TT; Knijnenburg J; Lommerse AAJ; van der Smagt JJ; Bezzina CR; Vandenberg JI; Verkerk AO; Barge-Schaapveld DQCM; Lodder EM, 2023, 'Reclassification of a likely pathogenic Dutch founder variant in KCNH2; implications of reduced penetrance', Human Molecular Genetics, 32, pp. 1072 - 1082, http://dx.doi.org/10.1093/hmg/ddac261

G J; Vandenberg JI; Ng C-A, 2023, 'Development of automated patch clamp assays to overcome the burden of variants of uncertain significance in inheritable arrhythmia syndromes', Frontiers in Physiology, 14, pp. 1294741, http://dx.doi.org/10.3389/fphys.2023.1294741

Jiang C; Richardson E; Farr J; Hill AP; Ullah R; Kroncke BM; Harrison SM; Thomson KL; Ingles J; Vandenberg JI; Ng CA, 2022, 'A calibrated functional patch-clamp assay to enhance clinical variant interpretation in KCNH2-related long QT syndrome', American Journal of Human Genetics, 109, pp. 1199 - 1207, http://dx.doi.org/10.1016/j.ajhg.2022.05.002

Ng CA; Ullah R; Farr J; Hill AP; Kozek KA; Vanags LR; Mitchell DW; Kroncke BM; Vandenberg JI, 2022, 'A massively parallel assay accurately discriminates between functionally normal and abnormal variants in a hotspot domain of KCNH2', American Journal of Human Genetics, 109, pp. 1208 - 1216, http://dx.doi.org/10.1016/j.ajhg.2022.05.003

Ng CA; Vandenberg JI, 2022, 'When it takes two to get one into trouble', Heart Rhythm, 19, pp. 293 - 294, http://dx.doi.org/10.1016/j.hrthm.2021.10.017

Li JV; Ng CA; Cheng D; Zhou Z; Yao M; Guo Y; Yu ZY; Ramaswamy Y; Ju LA; Kuchel PW; Feneley MP; Fatkin D; Cox CD, 2021, 'Modified N-linked glycosylation status predicts trafficking defective human Piezo1 channel mutations', Communications Biology, 4, http://dx.doi.org/10.1038/s42003-021-02528-w

Ng CA; Farr J; Young P; Windley MJ; Perry MD; Hill AP; Vandenberg JI, 2021, 'Heterozygous KCNH2 variant phenotyping using Flp-In HEK293 and high-Throughput automated patch clamp electrophysiology', Biology Methods and Protocols, 6, pp. 1 - 9, http://dx.doi.org/10.1093/biomethods/bpab003

Kozek KA; Glazer AM; Ng CA; Blackwell D; Egly CL; Vanags LR; Blair M; Mitchell D; Matreyek KA; Fowler DM; Knollmann BC; Vandenberg JI; Roden DM; Kroncke BM, 2020, 'High-throughput discovery of trafficking-deficient variants in the cardiac potassium channel KV11.1', Heart Rhythm, 17, pp. 2180 - 2189, http://dx.doi.org/10.1016/j.hrthm.2020.05.041

Perry MD; Ng CA; Mangala MM; Ng TYM; Hines AD; Liang W; Xu MJO; Hill AP; Vandenberg JI, 2020, 'Pharmacological activation of IKrin models of long QT Type 2 risks overcorrection of repolarization', Cardiovascular Research, 116, pp. 1434 - 1445, http://dx.doi.org/10.1093/cvr/cvz247

Ng CA; Perry MD; Liang W; Smith NJ; Foo B; Shrier A; Lukacs GL; Hill AP; Vandenberg JI, 2020, 'High-throughput phenotyping of heteromeric human ether-à-go-go-related gene potassium channel variants can discriminate pathogenic from rare benign variants', Heart Rhythm, 17, pp. 492 - 500, http://dx.doi.org/10.1016/j.hrthm.2019.09.020

Phan K; Ng CA; David E; Shishmarev D; Kuchel PW; Vandenberg JI; Perry MD, 2017, 'The S1 helix critically regulates the finely tuned gating of Kv11.1 channels', Journal of Biological Chemistry, 292, pp. 7688 - 7705, http://dx.doi.org/10.1074/jbc.M117.779298

Ng CA; Gravel AE; Perry MD; Arnold AA; Marcotte I; Vandenberg JI, 2016, 'Tyrosine residues from the s4-s5 linker of kv11.1 channels are critical for slow deactivation', Journal of Biological Chemistry, 291, pp. 17293 - 17302, http://dx.doi.org/10.1074/jbc.M116.729392

Perry MD; Ng CA; Phan K; David E; Steer K; Hunter MJ; Mann SA; Imtiaz M; Hill AP; Ke Y; Vandenberg JI, 2016, 'Rescue of protein expression defects may not be enough to abolish the pro-arrhythmic phenotype of long QT type 2 mutations', Journal of Physiology, 594, pp. 4031 - 4049, http://dx.doi.org/10.1113/JP271805

Cox CD; Bae C; Ziegler L; Hartley S; Nikolova-Krstevski V; Rohde PR; Ng CA; Sachs F; Gottlieb PA; Martinac B, 2016, 'Removal of the mechanoprotective influence of the cytoskeleton reveals PIEZO1 is gated by bilayer tension', Nature Communications, 7, http://dx.doi.org/10.1038/ncomms10366

Perry MD; Ng CA; Mann SA; Sadrieh A; Imtiaz M; Hill AP; Vandenberg JI, 2015, 'Getting to the heart of hERG K⁺ channel gating', Journal of Physiology, 593, pp. 2575 - 2585, http://dx.doi.org/10.1113/JP270095

Ng CA; Phan K; Hill AP; Vandenberg JI; Perry MD, 2014, 'Multiple interactions between cytoplasmic domains regulate slow deactivation of Kv11.1 channels', Journal of Biological Chemistry, 289, pp. 25822 - 25832, http://dx.doi.org/10.1074/jbc.M114.558379

Ke Y; Hunter MJ; Ng CA; Perry MD; Vandenberg JI, 2014, 'Role of the cytoplasmic N-terminal cap and Per-Arnt-Sim (PAS) domain in trafficking and stabilization of Kv11.1 channels', Journal of Biological Chemistry, 289, pp. 13782 - 13791, http://dx.doi.org/10.1074/jbc.M113.531277

Sadrieh A; Domanski L; Pitt-Francis J; Mann SA; Hodkinson EC; Ng CA; Perry MD; Taylor JA; Gavaghan D; Subbiah RN; Vandenberg JI; Hill AP, 2014, 'Multiscale cardiac modelling reveals the origins of notched T waves in long QT syndrome type 2', Nature Communications, 5, http://dx.doi.org/10.1038/ncomms6069

Ng CA; Ke Y; Perry MD; Tan PS; Hill AP; Vandenberg JI, 2013, 'C-Terminal β9-Strand of the Cyclic Nucleotide-Binding Homology Domain Stabilizes Activated States of Kv11.1 Channels', PLoS ONE, 8, pp. e77032, http://dx.doi.org/10.1371/journal.pone.0077032

Perry MD; Wong S; Ng CA; Vandenberg JI, 2013, 'Hydrophobic interactions between the voltage sensor and pore mediate inactivation in Kv11.1 channels', Journal of General Physiology, 142, pp. 275 - 288, http://dx.doi.org/10.1085/jgp.201310975

Ke Y; Ng CA; Hunter MJ; Mann SA; Heide J; Hill AP; Vandenberg JI, 2013, 'Trafficking defects in PAS domain mutant Kv11.1 channels: Roles of reduced domain stability and altered domain-domain interactions', Biochemical Journal, 454, pp. 69 - 77, http://dx.doi.org/10.1042/BJ20130328

Perry MD; Ng CA; Vandenberg JI, 2013, 'Pore helices play a dynamic role as integrators of domain motion during Kv11.1 channel inactivation gating', The Journal of Biological Chemistry, 288, pp. 11482 - 11491, http://dx.doi.org/10.1074/jbc.M113.461442

Vandenberg JI; Ng CA, 2012, 'Insights into hERG K+ channel structure and function from NMR studies', European Biophysics Journal With Biophysics Letters, 42, pp. 71 - 79, http://dx.doi.org/10.1007/s00249-012-0808-6

Ng CA; Perry M; Tan P; Hill A; Kuchel PW; Vandenberg JI, 2012, 'The S4-S5 linker acts as a signal integrator for HERG K+ channel activation and deactivation gating', PLoS ONE, 7, pp. e31640, http://dx.doi.org/10.1371/journal.pone.0031640

Tan PS; Perry MD; Ng C; Vandenberg JI; Hill A, 2012, 'Voltage-sensing domain mode shift is coupled to the activation gate by the N-terminal tail of hERG channels', Journal of General Physiology, 140, pp. 293 - 306, http://dx.doi.org/10.1085/jgp.201110761

Ng C; Hunter M; Perry M; Mobli M; Ke Y; Kuchel PW; King GF; Stock D; Vandenberg JI; Hunter M, 2011, 'The N-terminal tail of hERG contains an amphipathic alpha-helix that regulates channel deactivation', PLoS One, 6, pp. e16191, http://dx.doi.org/10.1371/journal.pone.0016191

Ng CA; Oehme DP; Kato Y; Tanokura M; Brownlee RTC, 2009, 'Binding of an RNA pol II ligand to the ww domain of Pin1 using molecular dynamics docking simulations', Journal of Chemical Theory and Computation, 5, pp. 2886 - 2897, http://dx.doi.org/10.1021/ct900190n

Ng CA; Kato Y; Tanokura M; Brownlee RTC, 2008, 'Structural characterisation of PinA WW domain and a comparison with other Group IV WW domains, Pin1 and Ess1', Biochimica et Biophysica Acta - Proteins and Proteomics, 1784, pp. 1208 - 1214, http://dx.doi.org/10.1016/j.bbapap.2008.04.026

Kato Y; Ng CA; Brownlee RTC; Tanokura M, 2007, 'PinA from Aspergillus nidulans binds to pS/pT-P motifs using the same Loop I and XP groove as mammalian Pin1', Biochimica et Biophysica Acta - Proteins and Proteomics, 1774, pp. 1208 - 1212, http://dx.doi.org/10.1016/j.bbapap.2007.06.006

Ng CA; Zhao W; Dang J; Bergdahl M; Separovic F; Brownlee RTC; Metzger RP, 2007, 'The conformation of acetylated virginiamycin M1 and virginiamycin M1 in explicit solvents', Biochimica et Biophysica Acta - Proteins and Proteomics, 1774, pp. 610 - 618, http://dx.doi.org/10.1016/j.bbapap.2007.03.002

Dang J; Metzger RP; Brownlee RTC; Ng CA; Bergdahl M; Separovic F, 2005, 'The conformational flexibility of the antibiotic virginiamycin M 1', European Biophysics Journal, 34, pp. 383 - 388, http://dx.doi.org/10.1007/s00249-005-0464-1

Conference Papers

Li J; Ng CA; Cheng D; Cox CD, 2021, 'Modified N-Linked Glycosylation Status Predicts Trafficking Defective', in BIOPHYSICAL JOURNAL, CELL PRESS, ELECTR NETWORK, pp. 236A - 237A, presented at 65th Annual Meeting of the Biophysical-Society (BPS), ELECTR NETWORK, 22 February 2021 - 26 February 2021, https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000629601401400&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=891bb5ab6ba270e68a29b250adbe88d1

Conference Abstracts

Lau C; Flood E; Hunter M; Corbett K; Ng C; Bouwer J; Stewart A; Perozo E; Allen TW; Vandenberg J, 2023, 'Structural basis of rapid inactivation of HERG potassium channels', in BIOPHYSICAL JOURNAL, CELL PRESS, Vol. 122, pp. 446A - 446A, https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000989629702422&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=891bb5ab6ba270e68a29b250adbe88d1

Ma G; O’Neill M; Richardson E; Thomson K; Ingles J; Roden D; Vandenberg J; Ng C; Glazer A, 2023, 'Utility of a High-Throughput Electrophysiology Assay to Determine Pathogenicity of SCN5A Variants Associated With Brugada Syndrome', in Heart, Lung and Circulation, Elsevier BV, Vol. 32, pp. S131 - S132, http://dx.doi.org/10.1016/j.hlc.2023.06.763

Ullah R; Ng CA; Vanags L; Mitchell D; Vandenberg J; Kroncke B, 2022, 'Abstract 12815: High Throughput Variant Identification in the S3-S5 Helices of KCNH2 (hERG)', in Circulation, Ovid Technologies (Wolters Kluwer Health), Vol. 146, http://dx.doi.org/10.1161/circ.146.suppl_1.12815

Jiang C; Richardson E; Farr J; Hill A; Ullah R; Kroncke B; Harrison S; Thomson K; Ingles J; Vandenberg J; Ng C, 2022, 'A Calibrated Functional Patch Clamp Assay to Enhance Clinical Variant Interpretation in KCNH2-Related Long QT Syndrome', in Heart Lung and Circulation, Elsevier, Vol. 31, pp. s37 - s37, presented at 70th Annual Scientific Meeting of the Cardiac Society of Australia and New Zealand, 11 August 2022 - 14 August 2022, http://dx.doi.org/10.1016/j.hlc.2022.06.003

Ullah R; Ng C; Farr J; Hill A; kozek K; Vanags L; Mitchell D; Vandenberg J; Kroncke B, 2021, 'Abstract 11153: Massively Parallel, High-Throughput Approach for Identifying Defective KCNH2 (hERG) Variants', in Circulation, Ovid Technologies (Wolters Kluwer Health), Vol. 144, http://dx.doi.org/10.1161/circ.144.suppl_1.11153

Jiang C; Farr J; Hill A; Kroncke B; Ingles J; Vandenberg J; Ng CA, 2021, 'Abstract 11857: Benchmarking a Functional Assay to Assist Clinical Classification of Variants of Uncertain Significance in KCNH2 (Long QT Syndrome Type 2)', in Circulation, Wolters Kluwer, Vol. 144, pp. a11857 - a11857, http://dx.doi.org/10.1161/circ.144.suppl_1.11857

Li J; Ann Ng C; Cheng D; Cox CD, 2021, 'Modified N-Linked Glycosylation Status Predicts Trafficking Defective Piezo1 Channel Mutations', in Biophysical Journal, Elsevier BV, Vol. 120, pp. 236a - 237a, http://dx.doi.org/10.1016/j.bpj.2020.11.1562

Cox CD; Ng CA; Bavi N; Martinac B, 2014, 'Mechanically-Induced Gating in PKD2L1 (Trpp2): Calcium-Induced Activation Masquerading as Force Sensitivity?', in BIOPHYSICAL JOURNAL, CELL PRESS, CA, San Francisco, Vol. 112, pp. 311A - 311A, presented at 58th Annual Meeting of the Biophysical-Society, CA, San Francisco, 15 February 2014 - 19 February 2014, http://dx.doi.org/10.1016/j.bpj.2016.11.1686

Tan PS; Perry M; Chai AN; Vandenberg JI; Hill AP, 2012, 'Bimodal Regulation of hERG Gating by the N-Terminal Tail Revealed by Voltage Clamp Fluorometry', in BIOPHYSICAL JOURNAL, CELL PRESS, CA, San Diego, Vol. 102, pp. 328A - 328A, presented at 56th Annual Meeting of the Biophysical-Society, CA, San Diego, 25 February 2012 - 29 February 2012, http://dx.doi.org/10.1016/j.bpj.2011.11.1800

Ke Y; Hunter M; Ng CA; Vandenberg J, 2012, 'Domain Misfolding and Destabilisation of Domain–Domain Interactions Both Contribute to Defective Trafficking of hERG EAG Domain Mutant Channels', in Heart, Lung and Circulation, Vol. 21, pp. 860 - 861, http://dx.doi.org/10.1016/j.hlc.2012.08.025

Phan K; Ng C; McNitt S; Moss A; Zareba W; Perry M; Vandenberg J, 'The role of in vitro phenotypes for clinical risk stratification of sudden death in Long QT Syndrome Type 2', in Heart, Lung and Circulation, Elsevier, Vol. 24, pp. S232 - S233, presented at Heart, Lung and Circulation, http://dx.doi.org/10.1016/j.hlc.2015.06.289

Datasets

Vandenberg J; Ng CA, 2016, Faculty Opinions recommendation of Tetrameric Assembly of K+ Channels Requires ER-Located Chaperone Proteins., , http://dx.doi.org/10.3410/f.727072588.793526218

Working Papers

Ma JG; O'Neill MJ; Richardson E; Thomson KL; Ingles J; Muhammad A; Solus JF; Davogustto G; Anderson KC; Benjamin Shoemaker M; Stergachis AB; Floyd BJ; Dunn K; Parikh VN; Chubb H; Perrin MJ; Roden DM; Vandenberg JI; Ng C-A; Glazer AM, 2023, Multi-site validation of a functional assay to adjudicate SCN5A Brugada Syndrome-associated variants., http://dx.doi.org10.1101/2023.12.19.23299592, https://www.ncbi.nlm.nih.gov/pubmed/38196587

Preprints

O'Neill MJ; Ng C-A; Aizawa T; Sala L; Bains S; Winbo A; Ullah R; Shen Q; Tan C-Y; Kozek K; Vanags LR; Mitchell DW; Shen A; Wada Y; Kashiwa A; Crotti L; Dagradi F; Musu G; Spazzolini C; Neves R; Bos JM; Giudicessi JR; Bledsoe X; Gamazon ER; Lancaster M; Glazer AM; Knollmann BC; Roden DM; Weile J; Roth F; Salem J-E; Earle N; Stiles R; Agee T; Johnson CN; Horie M; Skinner J; Ackerman MJ; Schwartz PJ; Ohno S; Vandenberg JI; Kroncke BM, 2024, Multiplexed Assays of Variant Effect and Automated Patch-clamping Improve KCNH2-LQTS Variant Classification and Cardiac Event Risk Stratification., , http://dx.doi.org/10.1101/2024.02.01.24301443

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