By Emeritus Professor John Whitelock

Book Chapters

Vassie JA; Whitelock JM; Lord MS, 2015, 'Glycosaminoglycan functionalized nanoparticles exploit glycosaminoglycan functions', in Balagurunathan K; Nakato H; Desai UR (ed.), Glycosaminoglycans, Springer Nature, pp. 557 - 565, http://dx.doi.org/10.1007/978-1-4939-1714-3_44

Estrella RP; Whitelock J; Roubin RH; Packer N; Karlsson N, 2009, 'Small-Scale Enzymatic Digestion of Glycoproteins and Proteoglycans for Analysis of Oligosaccharides by LC-MS and FACE Gel Electrophoresis', in Parker NH; Karlsson NG (ed.), Glycomics: Methods and Protocols (Methods in Molecular Biology), Volume 534, Humana Press, Inc, United States, pp. 171 - 192, http://dx.doi.org/10.1007/978-1-59745-022-5_13

Whitelock J; Melrose J, 2006, 'Adhesion of cells to biomaterials', in Metin Akay (ed.), Wiley Encyclopedia of Biomedical Engineering, John Wiley & Sons, USA, pp. 16 - 23

Melrose J; Whitelock J, 2006, 'Extracellular Matrix', in Metin Akay (ed.), Wiley Encyclopedia of Biomedical Engineering, John Wiley & Sons, USA, pp. 1556 - 1577

Whitelock J, 2001, 'Purification of Perlecan from Endothelial Cells', in Proteoglycan Protocols, Springer Nature, pp. 27 - 34, http://dx.doi.org/10.1385/1-59259-209-0:027

Journal articles

Jiang S; Yang N; Tan RP; Moh ESX; Fu L; Packer NH; Whitelock JM; Wise SG; Rnjak-Kovacina J; Lord MS, 2024, 'Tuning Recombinant Perlecan Domain V to Regulate Angiogenic Growth Factors and Enhance Endothelialization of Electrospun Silk Vascular Grafts', Advanced Healthcare Materials, 13, http://dx.doi.org/10.1002/adhm.202400855

Johnson BB; Cosson MV; Tsansizi LI; Holmes TL; Gilmore T; Hampton K; Song OR; Vo NTN; Nasir A; Chabronova A; Denning C; Peffers MJ; Merry CLR; Whitelock J; Troeberg L; Rushworth SA; Bernardo AS; Smith JGW, 2024, 'Perlecan (HSPG2) promotes structural, contractile, and metabolic development of human cardiomyocytes', Cell Reports, 43, http://dx.doi.org/10.1016/j.celrep.2023.113668

Guo Z; Zhuang C; Song Y; Yong J; Li Y; Guo Z; Kong B; Whitelock JM; Wang J; Liang K, 2023, 'Biocatalytic Buoyancy-Driven Nanobots for Autonomous Cell Recognition and Enrichment', Nano-Micro Letters, 15, http://dx.doi.org/10.1007/s40820-023-01207-1

Yong J; Hakobyan K; Xu J; Mellick AS; Whitelock J; Liang K, 2023, 'Comparison of protein quantification methods for protein encapsulation with ZIF-8 metal-organic frameworks', Biotechnology Journal, 18, http://dx.doi.org/10.1002/biot.202300015

Kim HN; Elgundi Z; Lin X; Fu L; Tang F; Moh ESX; Jung MS; Chandrasekar K; Bartlett-Tomasetig F; Foster C; Packer NH; Whitelock JM; Rnjak-Kovacina J; Lord MS, 2023, 'Engineered short forms of perlecan enhance angiogenesis by potentiating growth factor signalling', Journal of Controlled Release, 362, pp. 184 - 196, http://dx.doi.org/10.1016/j.jconrel.2023.08.052

Lau K; Fu L; Zhang A; Akhavan B; Whitelock J; Bilek MM; Lord MS; Rnjak-Kovacina J, 2023, 'Recombinant perlecan domain V covalently immobilized on silk biomaterials via plasma immersion ion implantation supports the formation of functional endothelium', Journal of Biomedical Materials Research - Part A, 111, pp. 825 - 839, http://dx.doi.org/10.1002/jbm.a.37525

Yong J; Mellick AS; Whitelock J; Wang J; Liang K, 2023, 'A Biomolecular Toolbox for Precision Nanomotors', Advanced Materials, 35, pp. e2205746, http://dx.doi.org/10.1002/adma.202205746

Chen K; Yong J; Zauner R; Wally V; Whitelock J; Sajinovic M; Kopecki Z; Liang K; Scott KF; Mellick AS, 2022, 'Chondroitin Sulfate Proteoglycan 4 as a Marker for Aggressive Squamous Cell Carcinoma', Cancers, 14, pp. 5564, http://dx.doi.org/10.3390/cancers14225564

Karimi F; Lau K; Kim HN; Och Z; Lim KS; Whitelock J; Lord M; Rnjak-Kovacina J, 2022, 'Surface Biofunctionalization of Silk Biomaterials Using Dityrosine Cross-Linking', ACS Applied Materials and Interfaces, 14, pp. 31551 - 31566, http://dx.doi.org/10.1021/acsami.2c03345

Mizumoto S; Kwok JCF; Whitelock JM; Li F; Perris R, 2022, 'Editorial: Roles of Chondroitin Sulfate and Dermatan Sulfate as Regulators for Cell and Tissue Development', Frontiers in Cell and Developmental Biology, 10, http://dx.doi.org/10.3389/fcell.2022.941178

Yong JM; Fu L; Tang F; Yu P; Kuchel RP; Whitelock JM; Lord MS, 2022, 'ROS-Mediated Anti-Angiogenic Activity of Cerium Oxide Nanoparticles in Melanoma Cells', ACS Biomaterials Science and Engineering, 8, pp. 512 - 525, http://dx.doi.org/10.1021/acsbiomaterials.1c01268

Hayes AJ; Whitelock J; Melrose J, 2022, 'Regulation of FGF-2, FGF-18 and Transcription Factor Activity by Perlecan in the Maturational Development of Transitional Rudiment and Growth Plate Cartilages and in the Maintenance of Permanent Cartilage Homeostasis', International Journal of Molecular Sciences, 23, http://dx.doi.org/10.3390/ijms23041934

Fu L; Li R; Whitelock JM; Lord MS, 2022, 'Tuning the intentional corona of cerium oxide nanoparticles to promote angiogenesis via fibroblast growth factor 2 signalling', Regenerative Biomaterials, 9, http://dx.doi.org/10.1093/rb/rbac081

Lau K; Waterhouse A; Akhavan B; Gao L; Kim HN; Tang F; Whitelock JM; Bilek MM; Lord MS; Rnjak-Kovacina J, 2021, 'Biomimetic silk biomaterials: Perlecan-functionalized silk fibroin for use in blood-contacting devices', Acta Biomaterialia, 132, pp. 162 - 175, http://dx.doi.org/10.1016/j.actbio.2021.02.014

Chandrasekar K; Farrugia BL; Johnson L; Marks D; Irving D; Elgundi Z; Lau K; Kim HN; Rnjak-Kovacina J; Bilek MM; Whitelock JM; Lord MS, 2021, 'Effect of Recombinant Human Perlecan Domain V Tethering Method on Protein Orientation and Blood Contacting Activity on Polyvinyl Chloride', Advanced Healthcare Materials, 10, http://dx.doi.org/10.1002/adhm.202100388

Ng CY; Whitelock JM; Williams H; Kim HN; Medbury HJ; Lord MS, 2021, 'Macrophages bind LDL using heparan sulfate and the perlecan protein core', Journal of Biological Chemistry, 296, pp. 100520, http://dx.doi.org/10.1016/j.jbc.2021.100520

Liu J; Guo Z; Li Y; Liang J; Xue J; Xu J; Whitelock JM; Xie L; Kong B; Liang K, 2021, 'pH-Gated Activation of Gene Transcription and Translation in Biocatalytic Metal–Organic Framework Artificial Cells', Advanced NanoBiomed Research, 1, http://dx.doi.org/10.1002/anbr.202000034

Lord MS; Melrose J; Day AJ; Whitelock JM, 2020, 'The Inter-α-Trypsin Inhibitor Family: Versatile Molecules in Biology and Pathology', Journal of Histochemistry and Cytochemistry, 68, pp. 907 - 927, http://dx.doi.org/10.1369/0022155420940067

Lin X; Tang F; Jiang S; Khamis H; Bongers A; Whitelock JM; Lord MS; Rnjak-Kovacina J, 2020, 'A Biomimetic Approach toward Enhancing Angiogenesis: Recombinantly Expressed Domain V of Human Perlecan Is a Bioactive Molecule That Promotes Angiogenesis and Vascularization of Implanted Biomaterials', Adv. Sci., 7, pp. 2000900, http://dx.doi.org/10.1002/advs.202000900

Lord M; Whitelock J; Turnbull JE, 2020, 'Better growth-factor binding aids tissue repair', Nature Biomedical Engineering, 4, pp. 368 - 369, http://dx.doi.org/10.1038/s41551-020-0548-3

Tang F; Manz XD; Bongers A; Odell RA; Joukhdar H; Whitelock JM; Lord MS; Rnjak-Kovacina J, 2020, 'Microchannels are an architectural cue that promotes integration and vascularization of silk biomaterials in vivo', ACS Biomaterials Science and Engineering, 6, pp. 1476 - 1486, http://dx.doi.org/10.1021/acsbiomaterials.9b01624

Elgundi Z; Papanicolaou M; Major G; Cox TR; Melrose J; Whitelock JM; Farrugia BL, 2020, 'Cancer Metastasis: The Role of the Extracellular Matrix and the Heparan Sulfate Proteoglycan Perlecan', Frontiers in Oncology, 9, pp. 1482, http://dx.doi.org/10.3389/fonc.2019.01482

Vennin C; Mélénec P; Rouet R; Nobis M; Cazet AS; Murphy KJ; Herrmann D; Reed DA; Lucas MC; Warren SC; Elgundi Z; Pinese M; Kalna G; Roden D; Samuel M; Zaratzian A; Grey ST; Da Silva A; Leung W; Johns AL; Chantrill LA; Chou A; Steinmann A; Arshi M; Dwarte T; Froio D; Pereira B; Ritchie S; Chambers CR; Metcalf X; Waddell N; Pearson JV; Patch AM; Nones K; Newell F; Mukhopadhyay P; Addala V; Kazakoff S; Holmes O; Leonard C; Wood S; Grimmond SM; Hofmann O; Christ A; Bruxner T; Samra JS; Pavlakis N; High HA; Asghari R; Merrett ND; Pavey D; Das A; Cosman PH; Ismail K; O’Connnor C; Stoita A; Williams D; Spigellman A; Lam VW; McLeod D; Kirk J; Kench JG; Grimison P; Cooper CL; Sandroussi C; Goodwin A; Mead RS; Tucker K; Andrews L; Texler M; Forest C; Epari KP; Ballal M; Fletcher DR; Mukhedkar S; Zeps N; Beilin M; Feeney K; Nguyen NQ; Ruszkiewicz AR; Worthley C; Chen J; Brooke-Smith ME; Papangelis V; Clouston AD; Barbour AP; O’Rourke TJ; Fawcett JW; Slater K; Hatzifotis M; Hodgkinson P; Nikfarjam M; Eshleman JR; Hruban RH; Wolfgang CL; Lawlor RT; Beghelli S; Corbo V; Scardoni M; Bassi C; Christ D; Cox T; Timpson P; Phillips P; Whitelock J; Pajic M; Gill A; Croucher D, 2019, 'CAF hierarchy driven by pancreatic cancer cell p53-status creates a pro-metastatic and chemoresistant environment via perlecan', Nature Communications, 10, pp. 3637, http://dx.doi.org/10.1038/s41467-019-10968-6

Farrugia BL; Mizumoto S; Lord MS; O’Grady RL; Kuchel RP; Yamada S; Whitelock JM, 2019, 'Hyaluronidase-4 is produced by mast cells and can cleave serglycin chondroitin sulfate chains into lower molecular weight forms', Journal of Biological Chemistry, 294, pp. 11458 - 11472, http://dx.doi.org/10.1074/jbc.RA119.008647

Gunatillake T; Chui A; Fitzpatrick E; Ignjatovic V; Monagle P; Whitelock J; Zanten D; Eijsink J; Borg A; Stevenson J; Brennecke SP; Erwich JJHM; Said JM; Murthi P, 2019, 'Decreased placental glypican expression is associated with human fetal growth restriction', Placenta, 76, pp. 6 - 9, http://dx.doi.org/10.1016/j.placenta.2018.12.007

Lord MS; Tang F; Rnjak-Kovacina J; Smith JGW; Melrose J; Whitelock JM, 2018, 'The multifaceted roles of perlecan in fibrosis', Matrix Biology, 68-69, pp. 150 - 166, http://dx.doi.org/10.1016/j.matbio.2018.02.013

Farrugia B; Mi Y; Kim HN; Whitelock J; Baker S; Wiesmann W; Li Z; Maitz P; Lord M, 2018, 'Chitosan‐Based Heparan Sulfate Mimetics Promote Epidermal Formation in a Human Organotypic Skin Model', Advanced Functional Materials, 28, http://dx.doi.org/10.1002/adfm.201802818

Farrugia BL; Lord MS; Whitelock JM; Melrose J, 2018, 'Harnessing chondroitin sulphate in composite scaffolds to direct progenitor and stem cell function for tissue repair', Biomaterials Science, 6, pp. 947 - 957, http://dx.doi.org/10.1039/c7bm01158j

Farrugia BL; Lord MS; Melrose J; Whitelock JM, 2018, 'The Role of Heparan Sulfate in Inflammation, and the Development of Biomimetics as Anti-Inflammatory Strategies', Journal of Histochemistry and Cytochemistry, 66, pp. 321 - 336, http://dx.doi.org/10.1369/0022155417740881

Lord M; Rnjak-Kovacina J; Tang F; Whitelock J, 2018, 'Glycosaminoglycan and Proteoglycan-Based Biomaterials: Current Trends and Future Perspectives', Advanced Healthcare Materials, 7, pp. 1701042 - 1701042, http://dx.doi.org/10.1002/adhm.201701042

Vassie JA; Whitelock JM; Lord MS, 2018, 'Targeted Delivery and Redox Activity of Folic Acid-Functionalized Nanoceria in Tumor Cells', Molecular Pharmaceutics, 15, pp. 994 - 1004, http://dx.doi.org/10.1021/acs.molpharmaceut.7b00920

Tang F; Lord M; Stallcup W; Whitelock J, 2018, 'Cell surface chondroitin sulfate proteoglycan 4 (CSPG4) binds to the basement membrane heparan sulfate proteoglycan, perlecan, and is involved in cell adhesion', The Journal of Biochemistry

Hayes A; Sugahara K; Farrugia B; Whitelock JM; Caterson B; Melrose J, 2018, 'Biodiversity of CS–proteoglycan sulphation motifs: Chemical messenger recognition modules with roles in information transfer, control of cellular behaviour and tissue morphogenesis', Biochemical Journal, 475, pp. 587 - 620, http://dx.doi.org/10.1042/BCJ20170820

Rnjak-Kovacina J; Tang F; Lin X; Whitelock JM; Lord MS, 2017, 'Recombinant Domain V of Human Perlecan Is a Bioactive Vascular Proteoglycan', Biotechnology Journal, 12, http://dx.doi.org/10.1002/biot.201700196

Lord MS; Jung MS; Whitelock JM, 2017, 'Optimization of bioengineered heparin/heparan sulfate production for therapeutic applications', Bioengineered, 8, pp. 661 - 664, http://dx.doi.org/10.1080/21655979.2017.1301328

Chui AKL; Gunatillake TN; Ignjatovic V; Monagle PT; Murthi P; Brennecke SP; Whitelock JM; Said JM, 2017, 'Antiangiogenic effects of decorin restored by unfractionated, low molecular weight, and nonanticoagulant heparins', Blood Advances, 1, pp. 1243 - 1253, http://dx.doi.org/10.1182/bloodadvances.2017004333

Chui A; Gunatillake T; Brennecke SP; Ignjatovic V; Monagle PT; Whitelock JM; Van Zanten DE; Eijsink J; Wang Y; Deane J; Borg AJ; Stevenson J; Erwich JJ; Said JM; Murthi P, 2017, 'Expression of Biglycan in First Trimester Chorionic Villous Sampling Placental Samples and Altered Function in Telomerase-Immortalized Microvascular Endothelial Cells', Arteriosclerosis, Thrombosis, and Vascular Biology, 37, pp. 1168 - 1179, http://dx.doi.org/10.1161/ATVBAHA.117.309422

Kim HN; Whitelock JM; Lord MS, 2017, 'Structure-activity relationships of bioengineered heparin/heparan sulfates produced in different bioreactors', Molecules, 22, pp. 806, http://dx.doi.org/10.3390/molecules22050806

Lord MS; Ellis AL; Farrugia BL; Whitelock JM; Grenett H; Li C; O'Grady RL; DeCarlo AA, 2017, 'Perlecan and vascular endothelial growth factor-encoding DNA-loaded chitosan scaffolds promote angiogenesis and wound healing', Journal of Controlled Release, 250, pp. 48 - 61, http://dx.doi.org/10.1016/j.jconrel.2017.02.009

Lord MS; Cheng B; Farrugia BL; McCarthy S; Whitelock JM, 2017, 'Platelet factor 4 binds to vascular proteoglycans and controls both growth factor activities and platelet activation', Journal of Biological Chemistry, 292, pp. 4054 - 4063, http://dx.doi.org/10.1074/jbc.M116.760660

Vassie JA; Whitelock JM; Lord MS, 2017, 'Endocytosis of cerium oxide nanoparticles and modulation of reactive oxygen species in human ovarian and colon cancer cells', Acta Biomaterialia, 50, pp. 127 - 141, http://dx.doi.org/10.1016/j.actbio.2016.12.010

Krilis M; Qi M; Madigan MC; Wong JWH; Abdelatti M; Guymer RH; Whitelock J; McCluskey P; Zhang P; Qi J; Hunyor AP; Krilis SA; Giannakopoulos B, 2017, 'Nitration of tyrosines in complement factor H domains alters its immunological activity and mediates a pathogenic role in age related macular degeneration', Oncotarget, 8, pp. 49016 - 49032, http://dx.doi.org/10.18632/oncotarget.14940

Rnjak-Kovacina J; Tang F; Whitelock JM; Lord MS, 2016, 'Silk biomaterials functionalized with recombinant domain V of human perlecan modulate endothelial cell and platelet interactions for vascular applications', Colloids and Surfaces B: Biointerfaces, 148, pp. 130 - 138, http://dx.doi.org/10.1016/j.colsurfb.2016.08.039

Lord MS; Cheng B; Tang F; Lyons JG; Rnjak-Kovacina J; Whitelock JM, 2016, 'Bioengineered human heparin with anticoagulant activity', Metabolic Engineering, 38, pp. 105 - 114, http://dx.doi.org/10.1016/j.ymben.2016.07.006

Lord MS; Farrugia BL; Yan CMY; Vassie JA; Whitelock JM, 2016, 'Hyaluronan coated cerium oxide nanoparticles modulate CD44 and reactive oxygen species expression in human fibroblasts', Journal of Biomedical Materials Research - Part A, 104, pp. 1736 - 1746, http://dx.doi.org/10.1002/jbm.a.35704

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