Tri studied graduated from medicine at the University of Sydney in 1994 and completed a double fellowship in Internal Medicine and Pathology in 2002 under the guidance Dr Stephen Adelstein and Dr Roger Garsia in the Department of Clinical Immunology at the Royal Prince Alfred Hospital, Sydney. For his PhD he developed a B cell receptor knock-in mouse model to study in vivo B cell responses to foreign and self-antigen under the supervision of Prof Antony Basten and Prof Robert Brink at the Centenary Institute, Sydney.

His interest in defining the in vivo contexts of B cell responses and resolving germinal centre selection events in space and time lead to post-doctoral studies with Prof Jason Cyster at the Howard Hughes Medical Institute, University of California, San Francisco where he used intravital two-photon microscopy to investigate the initiation of B cell responses in the lymph node. Tri established an intravital two-photon microscope facility at the Garvan Institute in 2010. His lab combines in vivo optical marking, single cell transcriptomics and CRISPR/Cas9 technologies to track the origin and fate of cells critical to immune responses in infection, autoimmunity and cancer.